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Viroids are the smallest known plant pathogens and consist of naked circular single-stranded RNA, ranging in size from to nucleotides. In addition, CEVd was reported to persist for a long period as an infectious agent on contaminated surfaces, dry infected host tissue and is highly resistant to heat and several viral disinfectants. For these reasons, prevention, control and eradication are hardly successful. Both China and Cambodia are exporting a huge amount of orange and lime fruits, respectively, to Thailand. To be able to conduct a risk assessment and determine management steps, a survey on the presence of the four above-mentioned viroids was set up at the points of entry in Thailand.

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For the Cambodian lime fruits, all samplings were conducted at the markets near the border between two countries Chanthaburi, Aranyaprathet, Chon Buri, Phetchabun and Sa Kaeo sites. All the samples were also tested for the internal control of the detection using NAD primer pair [ 4 ]. All primers were used at a final concentration of 0. All individual sequences were submitted to GenBank Accn.

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The results show that the contamination rate of these two viroids in both fruit samples is quite high, especially in orange samples, being Unfortunately, there is no information about the likelihood of establishment and spread of these diseases, impact and consequence to the orange and lime production in Thailand, or even for other economically important crops.

In a next step, all data will be used to update phytosanitary status and measures and will be useful for future risk assessment studies.

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Read article at publisher's site DOI : To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation. Plant Dis , 93 8 , 01 Aug Cited by: 0 articles PMID: Plant Dis , 92 6 , 01 Jun Plant Dis , 94 7 , 01 Jul Semancik JS , Vidalakis G.

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Arch Virol , 6 , 13 Apr Cited by: 9 articles PMID: J Virol Methods , , 07 Apr Europe PMC requires Javascript to function effectively. Each entry you wish to retrieve MUST be on a new line and in the format: "database name":"id" e. ENA Sequence. Sequence formats are simply the way in which the amino acid or DNA sequence is recorded in a computer file.


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Different programs expect different formats, so if you are to submit a job successfully, it is important to understand what the various formats look like. The first word on this line is the name of the sequence. The rest of the line is a description of the sequence. If no format is specified, as in an http request, the default format will be used.

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