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Once complete, the portrait's history was a mystery, until it was acquired by a Sardinian king in for the Royal Library of Turin. For decades in the 20th century, it was framed and displayed publicly, where it was exposed to sunlight, study co-author Mauro Missori, a researcher at the National Research Council in Italy, told Live Science in an email.

Centuries of poor conditions have led to the characteristic yellowing that is so common in ancient works of art. The yellowing reduces contrast with the red-chalk of the painting, making the image seem to vanish over time. As a result, the portrait was too fragile for general viewing, and since has been locked away in a temperature- and humidity-controlled vault at the Royal Library of Turin. In , the authors got a rare chance to inspect the masterpiece. The researchers quantified the level of chromophores, or light-absorbing compounds, in the cellulose that forms the paper.

Secrets of Leonardo da Vinci | Genius, Codes, Mysteries & Theories

Chromophores preferentially absorb blue and violet light, and tend to scatter, or reflect, reddish and yellow-hued light, which gives old documents a yellowish tint. So the team measured how much light was reflected and absorbed from the ancient painting at different wavelengths of light , which correspond to different colors. They then compared those values with ones from modern and ancient pieces of paper aged in various conditions. The team found that the extreme yellowing likely resulted from the portrait's storage in a moist environment — perhaps a closed, dank space.

As a follow-up, they'd like to do a second analysis to see whether the painting is continuing to degrade, or whether its fading has stabilized now that it is kept in better conditions, Missori said.

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By quantifying the level of damage, the new study can help preservationists map out the best road to save this disappearing masterpiece. Other Editions 2.

Artworks and Artists of High Renaissance

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